These environmental factors should be carefully controlled to obtain consistent results. Note: In general, longevity assays require at least 100 flies, but a larger number (100–200 flies) is preferable because it is more likely to yield consistent results.ĬRITICAL: The lifespan of Drosophila is affected by temperature, diet, and many other factors. The genotypes of the flies are as follows: (Control): elav- GAL4/Y, (Aβ42): elav- GAL4/Y UAS- Aβ42/+. Experiments were repeated more than three times, and a representative result is shown ( Iijima et al., 2004). Median lifespans (Ages, d, days after eclosion Control 50 d, Aβ42 flies 29 d), p < 0.001 by Kaplan–Meier survival analyses with log-rank test (Control n = 216, Aβ42 flies n = 154). Survival curves of control and Aβ42 flies ( Iijima et al., 2004 Iijima and Chiang, 2008) Novex™ 10 to 20%, Tricine, 1.0 mm, Mini Protein Gel, 10-well Polyoxyethylene (10) octylphenyl ether (Triton X-100)ĮCL Prime Western Blotting Detection Reagents Potassium dihydrogen phosphate (KH 2PO 4) Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preabsorbed AntibodyĬhemicals, peptides, and recombinant proteinsįSB (1-fluoro-2,5-bis(3-carboxy-4-hydroxystyryl)benzene, 1% DMSO solution) Sheep Anti-Mouse IgG, Whole Ab ECL Antibody, HRP Conjugated Anti-β-amyloid, 1–16 antibody, clone 6E10 (mouse ascites)Īmyloid β (N) (82E1) Anti-Human Mouse IgG MoAb antibody
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